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  6. Witness HW Heartworm Canine/Feline Antigen Test Kit  (10 tests)


Witness HW Heartworm Canine/Feline Antigen Test Kit  (10 tests)

Item: 46711
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Heartworm Test Results In 10 Minutes Pet testing kits can be an easy and convenient way to confirm a pet diagnosis. Witness is a cutting edge manufacturer of a many types of pet test kits.

WITNESS HW Heartworm Canine/Feline Antigen Test Kit (10 tests) is an easy Rapid Immuno-Migration (RIM™) test that looks for the adult Dirofilaria immitis antigen that is associated with heartworm disease. It can be used with canine or feline plasma, serum or anticoagulated whole blood samples. It is easily manageable and only requires a small amount of contact with the animal.

Key Advantages

  • Straightforward test that needs only 15 seconds of animal contact
  • Quick results in as little as 10 minutes
  • No refrigeration required – test can be stored at room temperature
  • Easy on your pet – only requires 50 µl of sample.

Useful Information

View WITNESS® Heartworm DI Sheet.

Kit Contents

  • 10 or 25 pouches, each containing 1 test device.
  • 10 or 25 disposable pipettes.
  • 1 buffer dropper bottle (5.0 mL).
  • Instructions for use.


  • Do not use components after expiration date.
  • Refrigeration not required. Store the test kit at 2° to 25°C (35° to 77°F). Do not freeze.
  • Use the test within 30 minutes after opening the sealed pouch.
  • Avoid touching or damaging membrane at windows #1, #2, #3.
  • The WITNESS® device should be placed on a flat, horizontal surface while performing the test.
  • Use a separate pipette for each sample.
  • Hold pipette and buffer bottle vertically when dispensing sample and buffer.
  • Do not mix components of different serials.
  • Saline Buffer contains sodium azide, 0.05% (w/v), as a preservative.
  • For veterinary use only.

Test Procedure

Important: Allow sample and buffer drops to fall onto membrane at window #1. Do not touch pipette tip, sample or buffer drops, or buffer bottle tip directly to the membrane.

Sample application
  • Tear open the pouch provided and place the test device on a flat horizontal surface for the duration of the test.
  • Squeeze the provided pipette near the sealed end. Insert the open end of the pipette into an anticoagulated whole blood, serum, or plasma sample. Release the pressure slightly to draw up a small amount of sample into the pipette.
  • Holding the pipette vertically, transfer one drop of sample to the sample well, window #1. Allow sample to be completely absorbed.
Buffer dispensing
  • Remove the cap from the buffer bottle, hold it vertically and add two drops of buffer to the sample well window #1.
Reading test
  • Wait ten minutes, observe the presence or absence of pink/purple bands in reading windows #2 and #3.
  • Sample results are read in window #2. The control band is read in window #3.
    • Notes: The test is complete and may be read before 10 minutes if pink/purple bands are visible in both windows #2 and #3.
  • The presence of a pink/purple band only in window #3 before 10 minutes does not mean that the test is complete. A pink/purple band in window #2 may develop slower than the control pink/purple band in window #3.


  • Valid test: Test is validated if a pink/purple band is present in the reading window #3.
  • Negative for HW antigen: No band in reading window #2, with one pink/purple band in window #3.
  • Positive for HW antigen: One pink/purple band in reading window #2, with one pink/purple band in window #3.
Invalid Test
  • Invalid test: No pink/purple band in control window #3.

Note: A test result should always be interpreted in the context of all available clinical information and history for the dog or cat. Cats testing negative for heartworm antigen may benefit from other diagnostic tests, including heartworm antibody assays.


Customer Reviews

Review Summary
2 Reviews
100% (2)
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100% Recommend this product (2 of 2 responses)
By Jess
Witness heartworm test review
March 5, 2020
Awesome product. I keep all of my dogs on preventative year round but I still like to get them checked yearly and this makes it so much easier
By Cricket's M.
Simple, easy and affordable...
January 30, 2015
This test is simple and easy. If you can draw blood you can handle it without any issues. Local vets are now charging upwards to $35 per dog for HW testing and with10 dogs in my care the savings is profound. No snapping required. No storage in the no warm up time. Pull blood, run the test and results in minutes.

Questions and Answers

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